High performance liquid chromatography and atomic fluorescence spectrometric for determination of metal

 High performance liquid chromatography and atomic fluorescence spectrometric for determination of metal 

Atomic fluorescence spectrometry (AFS) is a spectroscopic technique developed in the seventy s, it by hydride generation technology, both to eliminate the matrix interference samples, simplify the instrument interface technology of HPLC and AFS, its price is also cheaper than ICP-MS a lot, and combined with technology can realize the speciation analysis of trace elements and is not only the total amount analysis. To establish a high performance liquid chromatography and atomic fluorescence spectrometry hyphenated technique can solve the above problems, not only can provide the convenient, reliable method for mercury compounds and arsenic compounds in environmental samples and biological samples in the determination of the shape, and can provide technical support for promotion of domestic atomic fluorescence, achieved remarkable social and economic benefits.

Research shows that, based on high performance liquid chromatography separation of various mercury compounds and arsenic compounds form, interface technology in combination with atomic fluorescence spectrometry is the key problem needed to be solved. High performance liquid chromatography and atomic fluorescence spectrometer is connected through a Teflon tube, middle is connected into the microwave digestion device (or UV light), organic mercury and arsenic compounds can greatly improve the conversion to inorganic mercury and arsenic compounds, improve its sensitivity. Optimization of inner diameter and length of Teflon tube can be a good separation effect.

With the development of scientific research and environmental protection departments for determination of metal form is more and more high requirements, the combined device will expand to more elemental speciation analysis, and by its high morphological separation ability, high sensitivity and performance price ratio and widely used.

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