Technical Articles
NANBEI Is Professional On Providing One-step Solution Of Laboratory Instruments And Equipment
Proper operation of an autoclave steam sterilizer is essential for achieving reliable sterilization results while ensuring operator safety and equipment longevity. Understanding standardized operating procedures helps prevent equipment damage, ensures consistent sterilization outcomes, and maintains compliance with medical and laboratory safety standards.
An autoclave is a sophisticated high-pressure vessel that utilizes steam under pressure to achieve sterilization temperatures above 100°C (212°F). By creating pressurized conditions, the high-pressure steam sterilizer raises water's boiling point, enabling temperatures of 121°C to 134°C that effectively eliminate bacteria, viruses, spores, and other microorganisms.
This sterilization method is particularly effective for:
Surgical instruments and medical devices
Laboratory glassware and equipment
Pharmaceutical containers and components
Heat-resistant materials and tools
Research equipment requiring sterile conditions
Nanbei Instruments specializes in manufacturing precision autoclave steam sterilizers that meet international safety and performance standards for medical, laboratory, and industrial applications.
Never rely exclusively on automatic water level controls. Manual monitoring prevents costly damage to heating elements:
Daily Inspection: Check water levels before each operating cycle
Manual Water Addition: Always power down the unit completely before adding water
Pressure Safety: Never open water inlet valves when the inter-layer is pressurized
Ventilation: Ensure vent valves remain open during water addition procedures
Critical Safety Rule: Never attempt to open the sterilization chamber while under pressure.
Keep the interlock handle secured during pressurized operations
Wait for complete pressure release before opening doors
Monitor pressure gauges throughout the sterilization cycle
Understand emergency pressure release procedures
Proper cooling protocols prevent thermal shock and ensure operator safety:
For Liquid Sterilization:
Release steam gradually using controlled venting
Allow natural cooling to prevent liquid boiling over
Wait minimum 10-15 minutes before opening chamber
For Solid Items:
Monitor internal temperature before door opening
Use appropriate personal protective equipment
Allow items to cool to safe handling temperatures
When power outages or equipment failures occur during sterilization cycles:
Temperature Assessment: Check if sterilization temperature was maintained
Timer Reset: Restart timing only after reaching proper sterilization temperature
Cycle Documentation: Record interruption details for quality assurance
Re-sterilization Decision: Consider complete cycle restart for critical items
Safety Valve Testing:
Test safety valve function multiple times weekly
Perform testing while inter-layer is under pressure
Document valve response and any irregularities
Replace valves showing delayed or incomplete operation
Interior Chamber Care:
Remove and clean storage trays daily
Wipe down chamber walls with appropriate disinfectants
Ensure complete moisture removal to prevent corrosion
Inspect door seals for wear or damage
Steam Generator Maintenance:
Drain pressure from generator hoses daily
Remove mineral deposits and scale buildup
Check for leaks in steam delivery systems
Monitor water quality and filtration systems
Outdoor Installation Requirements:
Construct weatherproof protective enclosures
Ensure adequate ventilation for heat dissipation
Install drainage systems for condensate management
Provide electrical protection from moisture intrusion
A professional high-pressure steam sterilizer from Nanbei Instruments incorporates several critical subsystems:
Sterilization Chamber:
Stainless steel construction for corrosion resistance
Precision-engineered door seals and locking mechanisms
Internal rack systems for optimal load arrangement
Temperature and pressure monitoring sensors
Advanced Control Systems:
Microprocessor-based cycle management
Digital temperature and pressure displays
Programmable cycle parameters
Data logging capabilities for validation
Safety and Protection Devices:
Multi-level water level protection systems
Over-temperature and over-pressure safety controls
Door interlock mechanisms
Emergency pressure relief systems
Heating and Steam Generation:
High-efficiency heating elements
Steam generator with automatic controls
Insulation systems for energy efficiency
Condensate recovery systems
Ideal for smaller facilities and mobile applications:
Compact design for limited space environments
Quick heating and cooling cycles
Manual or semi-automatic operation
Suitable for dental clinics and small laboratories
Perfect for laboratories with height restrictions:
Space-efficient vertical design
Multiple tray configurations
Advanced safety systems
Ideal for research facilities and medical offices
Designed for high-volume sterilization requirements:
Large capacity chambers
Automated loading systems available
Advanced process controls
Suitable for hospitals and industrial applications
Monthly Calibration Checks:
Temperature sensor accuracy verification
Pressure gauge calibration confirmation
Timer accuracy validation
Safety system functionality testing
Annual Professional Calibration:
Complete system performance validation
Replacement of calibration certificates
Software updates and system optimization
Comprehensive safety system inspection
Maintain detailed records for regulatory compliance:
Daily operation logs
Maintenance activity documentation
Calibration certificates and schedules
Incident reports and corrective actions
Symptom: Inconsistent sterilization temperaturesSolutions:
Check heating element function
Verify temperature sensor calibration
Inspect insulation integrity
Examine steam generation efficiency
Symptom: Pressure buildup or release issuesSolutions:
Test safety valve operation
Inspect door seal integrity
Check steam generator function
Verify pressure relief mechanisms
When choosing an autoclave steam sterilizer, consider these critical factors:
Capacity Requirements: Match chamber size to your sterilization volume needsApplication Specifics: Select features appropriate for your sterilization materialsSafety Standards: Ensure compliance with local and international regulationsMaintenance Support: Choose manufacturers like Nanbei Instruments with comprehensive service networks
Today's digital torque wrench testers and sterilization equipment incorporate sophisticated technologies:
Touchscreen control interfaces
Network connectivity for remote monitoring
Automated cycle optimization
Energy efficiency management
Predictive maintenance alerts
Standardized operation of autoclave steam sterilizers requires careful attention to safety protocols, environmental conditions, and maintenance procedures. By following these comprehensive guidelines and choosing quality equipment from established manufacturers like Nanbei Instruments, facilities can achieve consistent sterilization results while maximizing equipment lifespan and ensuring operator safety.
Regular training, proper maintenance, and adherence to standardized procedures form the foundation of successful sterilization programs. Invest in quality equipment and maintain rigorous operational standards to achieve optimal sterilization outcomes for your critical applications.